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Arabidopsis UBC 13 differentially regulates two programmed cell death pathways in responses to pathogen and low‐temperature stress
Author(s) -
Wang Lipu,
Wen Rui,
Wang Jinghe,
Xiang Daoquan,
Wang Qian,
Zang Yuepeng,
Wang Zheng,
Huang Shuai,
Li Xin,
Datla Raju,
Fobert Pierre R.,
Wang Hong,
Wei Yangdou,
Xiao Wei
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15435
Subject(s) - biology , mutant , effector , immune system , arabidopsis , phenotype , plant disease resistance , microbiology and biotechnology , gene , genetics
SummaryUBC 13 is required for Lys63‐linked polyubiquitination and innate immune responses in mammals, but its functions in plant immunity remain to be defined. Here we used genetic and pathological methods to evaluate roles of Arabidopsis UBC 13 in response to pathogens and environmental stresses. Loss of UBC 13 failed to activate the expression of numerous cold‐responsive genes and resulted in hypersensitivity to low‐temperature stress, indicating that UBC 13 is involved in plant response to low‐temperature stress. Furthermore, the ubc13 mutant displayed low‐temperature‐induced and salicylic acid‐dependent lesion mimic phenotypes. Unlike typical lesion mimic mutants, ubc13 did not enhance disease resistance against virulent bacterial and fungal pathogens, but diminished hypersensitive response and compromised effector‐triggered immunity against avirulent bacterial pathogens. UBC 13 differently regulates two types of programmed cell death in response to low temperature and pathogen. The lesion mimic phenotype in the ubc13 mutant is partially dependent on SNC 1 . UBC 13 interacts with an F‐box protein CPR 1 that regulates the homeostasis of SNC 1. However, the SNC 1 protein level was not altered in the ubc13 mutant, implying that UBC 13 is not involved in CPR 1‐regulated SNC 1 protein degradation. Taken together, our results revealed that UBC 13 is a key regulator in plant response to low temperature and pathogens.