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Interactive and noninteractive roles of histone H2B monoubiquitination and H3K36 methylation in the regulation of active gene transcription and control of plant growth and development
Author(s) -
Zhao Wei,
Neyt Pia,
Van Lijsebettens Mieke,
Shen WenHui,
Berr Alexandre
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15418
Subject(s) - biology , histone , chromatin , histone h3 , histone methyltransferase , histone methylation , transcription factor , epigenomics , genetics , regulation of gene expression , histone h2b , epigenetics , microbiology and biotechnology , dna methylation , gene , gene expression
SummaryCovalent modifications of histones are essential to control a wide range of processes during development and adaptation to environmental changes. With the establishment of reference epigenomes, patterns of histone modifications were correlated with transcriptionally active or silenced genes. These patterns imply the need for the precise and dynamic coordination of different histone‐modifying enzymes to control transcription at a given gene. Classically, the influence of these enzymes on gene expression is examined separately and their interplays rarely established. In Arabidopsis , HISTONE MONOUBIQUITINATION 2 ( HUB 2) mediates H2B monoubiquitination (H2Bub1), whereas SET DOMAIN GROUP 8 ( SDG 8) catalyzes H3 lysine 36 trimethylation (H3K36me3). In this work, we crossed hub2 with sdg8 mutants to elucidate their functional relationships. Despite similar phenotypic defects, sdg8 and hub2 mutations broadly affect genome transcription and plant growth and development synergistically. Also, whereas H3K4 methylation appears largely dependent on H2Bub1, H3K36me3 and H2Bub1 modifications mutually reinforce each other at some flowering time genes. In addition, SDG 8 and HUB 2 jointly antagonize the increase of the H3K27me3 repressive mark. Collectively, our data provide an important insight into the interplay between histone marks and highlight their interactive complexity in regulating chromatin landscape which might be necessary to fine‐tune transcription and ensure plant developmental plasticity.