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Virulence‐ and signaling‐associated genes display a preference for long 3′ UTR s during rice infection and metabolic stress in the rice blast fungus
Author(s) -
RodríguezRomero Julio,
Marconi Marco,
OrtegaCampayo Víctor,
Demuez Marie,
Wilkinson Mark D.,
Sesma Ane
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15405
Subject(s) - biology , gene , genetics , virulence , genome , polyadenylation , transposable element , untranslated region , podospora anserina , magnaporthe grisea , oryza sativa , mutant , gene expression , rna
Summary Generation of mRNA isoforms by alternative polyadenylation ( APA ) and their involvement in regulation of fungal cellular processes, including virulence, remains elusive. Here, we investigated genome‐wide polyadenylation site ( PAS ) selection in the rice blast fungus to understand how APA regulates pathogenicity. More than half of Magnaporthe oryzae transcripts undergo APA and show novel motifs in their PAS region. Transcripts with shorter 3′ UTR s are more stable and abundant in polysomal fractions, suggesting they are being translated more efficiently. Importantly, rice colonization increases the use of distal PAS s of pathogenicity genes, especially those participating in signalling pathways like 14‐3‐3B , whose long 3′ UTR is required for infection. Cleavage factor I ( CFI ) Rbp35 regulates expression and distal PAS selection of virulence and signalling‐associated genes, tRNA s and transposable elements, pointing its potential to drive genomic rearrangements and pathogen evolution. We propose a noncanonical PAS selection mechanism for Rbp35 that recognizes UGUAH , unlike humans, without CFI 25. Our results showed that APA controls turnover and translation of transcripts involved in fungal growth and environmental adaptation. Furthermore, these data provide useful information for enhancing genome annotations and for cross‐species comparisons of PAS s and PAS usage within the fungal kingdom and the tree of life.