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Rice PPS 1 encodes a DYW motif‐containing pentatricopeptide repeat protein required for five consecutive RNA ‐editing sites of nad3 in mitochondria
Author(s) -
Xiao Haijun,
Zhang Qiannan,
Qin Xiaojian,
Xu Yanghong,
Ni Chenzi,
Huang Jishuai,
Zhu Linlin,
Zhong Feiya,
Liu Wei,
Yao Guoxin,
Zhu Yingguo,
Hu Jun
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15347
Subject(s) - pentatricopeptide repeat , rna editing , biology , rna , rna binding protein , rna interference , cytoplasmic male sterility , mitochondrion , organelle , gene knockdown , genetics , computational biology , gene , microbiology and biotechnology
Summary The pentatricopeptide repeat ( PPR ) protein family is a large family characterized by tandem arrays of a degenerate 35‐amino‐acid motif whose members function as important regulators of organelle gene expression at the post‐transcriptional level. Despite the roles of PPR s in RNA editing in organelles, their editing activities and the underlying mechanism remain obscure. Here, we show that a novel DYW motif‐containing PPR protein, PPS 1, is associated with five conserved RNA ‐editing sites of nad3 located in close proximity to each other in mitochondria, all of which involve conversion from proline to leucine in rice. Both pps1 RNA i and heterozygous plants are characterized by delayed development and partial pollen sterility at vegetative stages and reproductive stage. RNA electrophoresis mobility shift assays ( REMSA s) and reciprocal competition assays using different versions of nad3 probes confirm that PPS 1 can bind to cis‐elements near the five affected sites, which is distinct from the existing mode of PPR ‐ RNA binding because of the continuity of the editing sites. Loss of editing at nad3 in pps1 reduces the activity of several complexes in the mitochondrial electron transport chain and affects mitochondrial morphology. Taken together, our results indicate that PPS 1 is required for specific editing sites in nad3 in rice.