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Chromatin remodeling factor CHR18 interacts with replication protein RPA1A to regulate the DNA replication stress response in Arabidopsis
Author(s) -
Han JiaJia,
Song ZeTing,
Sun JingLiang,
Yang ZhengTing,
Xian MengJun,
Wang Shuo,
Sun Ling,
Liu JianXiang
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15311
Subject(s) - biology , chromatin , dna replication , control of chromosome duplication , eukaryotic dna replication , origin recognition complex , nucleosome , chromatin remodeling , microbiology and biotechnology , replication factor c , pre replication complex , dna replication factor cdt1 , histone , arabidopsis , replication protein a , genetics , dna , dna binding protein , transcription factor , gene , mutant
Summary DNA replication is a fundamental process for the faithful transmission of genetic information in all living organisms. Many endogenous and environmental signals impede fork progression during DNA synthesis, which induces replication errors and DNA replication stress. Chromatin remodeling factors regulate nucleosome occupancy and the histone composition of the nucleosome in chromatin; however, whether chromatin remodeling factors are involved in the DNA replication stress response in plants is unknown. We reveal that chromatin remodeling factor CHR 18 plays important roles in DNA replication stress in Arabidopsis thaliana by interacting with the DNA replication protein RPA 1A. According to the genetic analysis, the loss of function of either CHR 18 or RPA 1A confers a high sensitivity to DNA replication stress in Arabidopsis. CHR 18 interacts with RPA 1A in both yeast cells and tobacco epidermal cells. The coexpression of RPA 1A and CHR 18 enhances the accumulation of CHR 18 in nuclear foci in plants. CHR 18 is a typical nuclear‐localized chromatin remodeling factor with ATP ase activity. Our results demonstrate that during DNA synthesis in plants, RPA 1A interacts with CHR 18 and recruits CHR 18 to nuclear foci to resolve DNA replication stress, which is important for cell propagation and root growth in Arabidopsis plants.