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Identification of Stipules reduced, a leaf morphology gene in pea ( Pisum sativum )
Author(s) -
Moreau Carol,
Hofer Julie M. I.,
Eléouët Morgane,
Sinjushin Andrey,
Ambrose Mike,
Skøt Kirsten,
Blackmore Tina,
Swain Martin,
Hegarty Matthew,
Balanzà Vicente,
Ferrándiz Cristina,
Ellis T. H. Noel
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15286
Subject(s) - stipule , biology , pisum , tendril , gene , sativum , mutant , botany , genetics
Summary Pea ( Pisum sativum ) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata , conferring stipule identity, was known to interact with Stipules reduced ( St ), which conditions stipule size in pea, but the St gene remained unknown. Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing. We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata . St regulates both cell division and cell expansion in the stipule. Our approach shows how systematic genome‐wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.