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A noncoding RNA transcribed from the AGAMOUS ( AG ) second intron binds to CURLY LEAF and represses AG expression in leaves
Author(s) -
Wu HuiWen,
Deng Shulin,
Xu Haiying,
Mao HuiZhu,
Liu Jun,
Niu QiWen,
Wang Huan,
Chua NamHai
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15231
Subject(s) - agamous , rna , intron , biology , repressor , non coding rna , psychological repression , microbiology and biotechnology , gene expression , arabidopsis , flowering locus c , rna binding protein , transcription (linguistics) , genetics , gene , mutant , linguistics , philosophy
Summary Dispersed H3K27 trimethylation (H3K27me3) of the AGAMOUS ( AG ) genomic locus is mediated by CURLY LEAF ( CLF ), a component of the Polycomb Repressive Complex ( PRC ) 2. Previous reports have shown that the AG second intron, which confers AG tissue‐specific expression, harbors sequences targeted by several positive and negative regulators. Using RACE reverse transcription polymerase chain reaction, we found that the AG intron 2 encodes several noncoding RNA s. RNA i experiment showed that inc RNA 4 is needed for CLF repressive activity. AG ‐inc RNA 4 RNA i lines showed increased leaf AG mRNA levels associated with a decrease of H3K27me3 levels; these plants displayed AG overexpression phenotypes. Genetic and biochemical analyses demonstrated that the AG ‐inc RNA 4 can associate with CLF to repress AG expression in leaf tissues through H3K27me3‐mediated repression and to autoregulate its own expression level. The mechanism of AG ‐inc RNA 4 ‐mediated repression may be relevant to investigations on tissue‐specific expression of Arabidopsis MADS ‐box genes.