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Os SPL regulates meiotic fate acquisition in rice
Author(s) -
Ren Lijun,
Tang Ding,
Zhao Tingting,
Zhang Fanfan,
Liu Changzhen,
Xue Zhihui,
Shi Wenqing,
Du Guijie,
Shen Yi,
Li Yafei,
Cheng Zhukuan
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15017
Subject(s) - meiosis , biology , transcription factor , microbiology and biotechnology , stamen , arabidopsis , callose , genetics , oryza sativa , cell fate determination , histone , mutant , chromatin , transcriptome , gene , botany , pollen , gene expression
Summary In angiosperms, the key step in sexual reproduction is successful acquisition of meiotic fate. However, the molecular mechanism determining meiotic fate remains largely unknown. Here, we report that OsSPOROCYTELESS (OsSPL) is critical for meiotic entry in rice ( Oryza sativa ). We performed a large‐scale genetic screen of rice sterile mutants aimed to identify genes regulating meiotic entry and identified Os SPL using map‐based cloning. We showed that meiosis‐specific callose deposition, chromatin organization, and centromere‐specific histone H3 loading were altered in the cells corresponding to pollen mother cells in Osspl anthers. Global transcriptome analysis showed that the enriched differentially expressed genes in Osspl were mainly related to redox status, meiotic process, and parietal cell development. Os SPL might form homodimers and interact with TEOSINTE BRANCHED1/CYCLOIDEA/PCF ( TCP ) transcription factor Os TCP 5 via the SPL dimerization and TCP interaction domain. Os SPL also interacts with TPL ( TOPLESS ) corepressors, Os TPL 2 and Os TPL 3, via the EAR motif. Our results suggest that the Os SPL ‐mediated signaling pathway plays a crucial role in rice meiotic entry, which appears to be a conserved regulatory mechanism for meiotic fate acquisition in angiosperms.