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Os CERK 1 plays a crucial role in the lipopolysaccharide‐induced immune response of rice
Author(s) -
Desaki Yoshitake,
Kouzai Yusuke,
Ninomiya Yusuke,
Iwase Ryosuke,
Shimizu Yumi,
Seko Keito,
Molinaro Antonio,
Minami Eiichi,
Shibuya Naoto,
Kaku Hanae,
Nishizawa Yoko
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14941
Subject(s) - arabidopsis , mutant , lipopolysaccharide , biology , microbiology and biotechnology , receptor , gene , immune system , immune receptor , c type lectin , gene knockout , innate immune system , biochemistry , genetics , immunology
Summary Plant cell surface receptor‐like kinases ( RLK s) mediate the signals from microbe‐associated molecular patterns ( MAMP s) that induce immune responses. Lipopolysaccharide ( LPS ), the major constituent of the outer membrane of gram‐negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co‐receptors are unknown except for LORE , a bulb‐type lectin S‐domain RLK (B‐lectin SD 1‐ RLK ) in Arabidopsis. Os CERK 1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP , and peptidoglycan, a bacterial MAMP . Here, we analyzed the relevance of Os CERK 1 to LPS perception in rice. Using Os CERK 1 ‐knockout mutants ( oscerk1 ), we evaluated hydrogen peroxide (H 2 O 2 ) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B‐lectin SD 1‐ RLK genes in rice and for all LysM‐protein genes in Arabidopsis. Compared with wild‐type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B‐lectin SD 1‐ RLK genes and Arabidopsis lines mutated in the LysM‐protein genes responded normally to LPS . From these results, we conclude that Os CERK 1 is an LPS receptor/co‐receptor and that the LPS perception systems of rice and Arabidopsis are significantly different.