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Evolution of micro RNA 827 targeting in the plant kingdom
Author(s) -
Lin WeiYi,
Lin YenYu,
Chiang SuFen,
Syu Cueihuan,
Hsieh LiChing,
Chiou TzyyJen
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14938
Subject(s) - biology , arabidopsis thaliana , arabidopsis , gene , genetics , brassicaceae , lineage (genetic) , microbiology and biotechnology , botany , mutant
Summary Unlike most ancient micro RNA s, which conservatively target homologous genes across species, micro RNA 827 (miR827) targets two different types of SPX ( SYG 1/ PHO 81/ XPR 1)‐domain‐containing genes, NITROGEN LIMITATION ADAPTATION ( NLA ) and PHOSPHATE TRANSPORTER 5 ( PHT 5 ), in Arabidopsis thaliana and Oryza sativa to regulate phosphate (Pi) transport and storage, respectively. However, how miR827 shifted its target preference and its evolutionary history are unknown. Based on target prediction analysis, we found that in most angiosperms, miR827 conservatively targets PHT 5 homologs, but in Brassicaceae and Cleomaceae it preferentially targets NLA homologs, and we provide evidence for the transition of target preference during Brassicales evolution. Intriguingly, we found a lineage‐specific loss of the miR827‐regulatory module in legumes. Analysis of miR827‐mediated cleavage efficiency and the expression of PHT 5 in A. thaliana indicated that accumulation of mutations in the target site and the exclusion of the target site by alternative transcriptional initiation eliminated PHT 5 targeting by miR827. Here, we identified a transition of miR827 target preference during plant evolution and revealed the uniqueness of miR827‐mediated regulation among conserved plant mi RNA s. Despite the change in its target preference, upregulation of miR827 by Pi starvation and its role in regulating cellular Pi homeostasis were retained.