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The iron‐regulated transporter 1 plays an essential role in uptake, translocation and grain‐loading of manganese, but not iron, in barley
Author(s) -
Long Lizhi,
Persson Daniel P.,
Duan Fengying,
Jørgensen Kirsten,
Yuan Lixing,
Schjoerring Jan K.,
Pedas Pai R.
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14930
Subject(s) - endodermis , hydroponics , arabidopsis , context (archaeology) , transporter , manganese , stele , shoot , biology , chromosomal translocation , arabidopsis thaliana , photosynthesis , microbiology and biotechnology , botany , biochemistry , chemistry , gene , mutant , paleontology , organic chemistry
Summary Transporters involved in manganese (Mn) uptake and intracellular Mn homeostasis in Arabidopsis and rice are well characterized, while much less is known for barley, which is particularly prone to Mn deficiency. In this study we have investigated the role of the iron‐regulated transporter 1 ( IRT 1) for Mn uptake and translocation in barley plants. We employed an RNA i approach to reduce Hv IRT 1 expression to 5% of the wild‐type level. This enabled characterization of the functional role of Hv IRT 1 by use of advanced imaging and phenotyping techniques applied to plants growing in hydroponics or soils with different Mn availability. Our results highlight the importance of Hv IRT 1 for the transport of Mn across the root endodermis into the stele. In the hvirt1 ‐ RNA i lines, a chlorotic phenotype with reduced shoot Mn concentration and impaired photosynthetic functionality was observed, especially under conditions with low Mn availability. We also document that Hv IRT 1 controlled the Mn distribution within the barley grain. Surprisingly, unlike other IRT 1 orthologues, Hv IRT 1 played no significant role in iron uptake. We conclude that the barley IRT 1 orthologue has a novel function with respect to ensuring sufficient shoot Mn concentrations. The preference of IRT 1 for Mn instead of Fe is discussed in an evolutionary context.