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Sustaining global agriculture through rapid detection and deployment of genetic resistance to deadly crop diseases
Author(s) -
Periyannan Sambasivam
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14928
Subject(s) - biology , genetics , cloning (programming) , gene , positional cloning , genome , microbiology and biotechnology , computational biology , mutant , computer science , programming language
ContentsSummary 45 I. Introduction 45 II. Targeted chromosome‐based cloning via long‐range assembly (TACCA) 46 III. Resistance gene cloning through mutational mapping (MutMap) 47 IV. Cloning through mutant chromosome sequencing (MutChromSeq) 47 V. Rapid cloning through resistance gene enrichment and sequencing (RenSeq) 49 VI. Cloning resistance genes through transcriptome profiling (RNAseq) 49 VII. Resistance gene deployment strategies 49 VIII. Conclusions 50Acknowledgements 50References 50Summary Genetically encoded resistance is a major component of crop disease management. Historically, gene loci conferring resistance to pathogens have been identified through classical genetic methods. In recent years, accelerated gene cloning strategies have become available through advances in sequencing, gene capture and strategies for reducing genome complexity. Here, I describe these approaches with key emphasis on the isolation of resistance genes to the cereal crop diseases that are an ongoing threat to global food security. Rapid gene isolation enables their efficient deployment through marker‐assisted selection and transgenic technology. Together with innovations in genome editing and progress in pathogen virulence studies, this creates further opportunities to engineer long‐lasting resistance. These approaches will speed progress towards a future of farming using fewer pesticides.