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Combined transcriptome and translatome analyses reveal a role for tryptophan‐dependent auxin biosynthesis in the control of DOG1 ‐dependent seed dormancy
Author(s) -
Bai Bing,
Novák Ondřej,
Ljung Karin,
Hanson Johannes,
Bentsink Leónie
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14885
Subject(s) - imbibition , transcriptome , germination , biology , auxin , dormancy , cordycepin , abscisic acid , seed dormancy , microbiology and biotechnology , desiccation , botany , biochemistry , gene expression , gene
Summary The importance of translational regulation during Arabidopsis seed germination has been shown previously. Here the role of transcriptional and translational regulation during seed imbibition of the very dormant DELAY OF GERMINATION 1 ( DOG1 ) near‐isogenic line was investigated. Polysome profiling was performed on dormant and after‐ripened seeds imbibed for 6 and 24 h in water and in the transcription inhibitor cordycepin. Transcriptome and translatome changes were investigated. Ribosomal profiles of after‐ripened seeds imbibed in cordycepin mimic those of dormant seeds. The polysome occupancy of mRNA species is not affected by germination inhibition, either as a result of seed dormancy or as a result of cordycepin treatment, indicating the importance of the regulation of transcript abundance. The expression of auxin metabolism genes is discriminative during the imbibition of after‐ripened and dormant seeds, which is confirmed by altered concentrations of indole‐3‐acetic acid conjugates and precursors.