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VirD5 is required for efficient Agrobacterium infection and interacts with Arabidopsis VIP2
Author(s) -
Wang Yafei,
Zhang Shaojuan,
Huang Fei,
Zhou Xu,
Chen Zhuo,
Peng Wei,
Luo Meizhong
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14854
Subject(s) - agrobacterium , arabidopsis , agrobacterium tumefaciens , transformation (genetics) , mutant , biology , virulence , carcinogenesis , microbiology and biotechnology , genetics , gene
Summary During Agrobacterium ( Agrobacterium tumefaciens ) infection, the translocated virulence proteins (VirD2, VirE2, VirE3, VirF and VirD5) play crucial roles. It is thought that, through protein–protein interactions, Agrobacterium uses and abuses host plant factors and systems to facilitate its infection. Although some molecular functions have been revealed, the roles of VirD5 still need to be further elucidated. Here, plant transformation and tumorigenesis mediated by genetically modified Agrobacterium strains were performed to examine VirD5 roles. In addition, protein–protein interaction‐associated molecular and biochemistry technologies were used to reveal and elucidate VirD5 interaction with Arabidopsis VirE2 interacting protein 2 (VIP2). Our results showed that deleting virD5 from Agrobacterium reduced its tumor formation ability and stable transformation efficiency but did not affect the transient transformation efficiency. We also found that VirD5 can interact with Arabidopsis VIP 2. Further experiments demonstrated that VirD5 can affect VIP 2 binding to cap‐binding proteins ( CBP 20 and CBP 80). The tumorigenesis efficiency for cbp80 mutant was not significantly changed, but that for cbp20 , cbp20cbp80 mutants were significantly increased. This work demonstrates experimentally that VirD5 is required for efficient Agrobacterium infection and may promote this process by competitive interaction with Arabidopsis VIP 2. CBP 20 is involved in the Agrobacterium infection process and its effect can be synergistically enhanced by CBP 80.