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Tolerance of olive ( Olea europaea ) cv Frantoio to Verticillium dahliae relies on both basal and pathogen‐induced differential transcriptomic responses
Author(s) -
LeyvaPérez María de la O,
JiménezRuiz Jaime,
GómezLama Cabanás Carmen,
ValverdeCorredor Antonio,
Barroso Juan B.,
Luque Francisco,
MercadoBlanco Jesús
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14833
Subject(s) - verticillium dahliae , biology , verticillium wilt , olea , transcriptome , plant disease resistance , pathogen , botany , gene , genetics , gene expression
Summary Verticillium wilt of olive ( VWO ) is one of the most serious biotic constraints for this tree crop. Our knowledge of the genetics of the tolerance/resistance to this disease is very limited. Here we show that tolerance of the cv Frantoio relies on both basal and early pathogen‐induced differential transcriptomic responses. A comparative transcriptomic analysis ( RNA ‐seq) was conducted in root tissues of cvs Frantoio ( VWO ‐tolerant) and Picual ( VWO ‐susceptible). RNA samples originated from roots of inoculated olive plants during the early infection stages by Verticillium dahliae (highly virulent, defoliating pathotype). A huge number of differentially expressed genes ( DEG s) were found between ‘Frantoio’ and ‘Picual’ (27 312 unigenes) in the absence of the pathogen. Upon infection with V. dahliae , ‘Picual’ and ‘Frantoio’ plants responded differently too. In the early infection stages, four clusters of DEG s could be identified according to their time‐course expression patterns. Among others, a pathogenesis‐related protein of the Bet v I family and a dirigent‐like protein involved in lignification, and several BAK 1 , NHL 1 , reactive oxygen species stress response and BAM unigenes showed noticeable differences between cultivars. Tolerance of ‘Frantoio’ plants to VWO is a consequence of a complex and multifaceted process which involves many plant traits.