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DNA METHYLTRANSFERASE 1 ‐mediated shoot regeneration is regulated by cytokinin‐induced cell cycle in Arabidopsis
Author(s) -
Liu Hui,
Zhang Hui,
Dong Yu Xiu,
Hao Yu Jin,
Zhang Xian Sheng
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14814
Subject(s) - dna methylation , biology , arabidopsis , cytokinin , microbiology and biotechnology , chromatin immunoprecipitation , chromatin , cell cycle , shoot , methylation , genetics , gene expression , dna , botany , cell , gene , auxin , mutant , promoter
SummaryDNA methylation plays a critical role in diverse biological processes of plants. Arabidopsis DNA METHYLTRANSFERASE 1 ( MET 1) represses shoot regeneration by inhibiting WUSCHEL ( WUS ) expression, which is essential for shoot initiation. However, the upstream signals regulating MET 1 expression during this process are unclear. We analyzed the signals regulating MET 1 expression using a number of established strategies, such as genetic analysis, confocal microscopy, quantitative real‐time PCR and chromatin immunoprecipitation. MET 1 expression patterns underwent dynamic changes with the initiation of WUS during shoot regeneration. The cell cycle regulator E2 FA was characterized as an upstream factor directly promoting MET 1 expression. Moreover, cytokinin promoted MET 1 expression partially by enhancing CYCD 3 expression. Our findings reveal that MET 1 ‐mediated shoot regeneration is regulated by the cytokinin‐induced cell cycle, and provide new insights into the regulation of DNA methylation in shoot regeneration.