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NRAMP2, a trans‐Golgi network‐localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency
Author(s) -
Gao Huiling,
Xie Wenxiang,
Yang Changhong,
Xu Jingyi,
Li Jingjun,
Wang Hua,
Chen Xi,
Huang ChaoFeng
Publication year - 2018
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14783
Subject(s) - arabidopsis , mutant , golgi apparatus , arabidopsis thaliana , chemistry , root hair , mutation , microbiology and biotechnology , subcellular localization , reactive oxygen species , biochemistry , biology , gene , endoplasmic reticulum
Summary To cope with manganese (Mn) deficiency, plants have evolved an efficient transport system to uptake and redistribute Mn. However, the underlying molecular mechanisms remain to be demonstrated. We carried out a forward genetic screen in a root high‐affinity Mn transporter nramp1 mutant background in Arabidopsis thaliana and identified an uncharacterized Mn transport NRAMP 2. We investigated the effect of nramp2 mutation on root growth and reactive oxygen species ( ROS ) accumulation and we also examined the NRAMP 2 expression pattern, and the subcellular localization and transport activity of NRAMP 2. Mutation of NRAMP2 impaired plant growth, while overexpression of NRAMP2 improved plant growth under low Mn conditions. In the nramp2‐1nramp1 double mutant, Mn deficiency inhibited root cell elongation and root hair development, which was associated with increased hydrogen peroxide (H 2 O 2 ) accumulation. NRAMP 2 is preferentially localized to the trans‐Golgi network. NRAMP 2 has Mn influx transport activity in yeast, and mutation of NRAMP2 led to greater Mn retention in roots. Our results suggest that under Mn‐deficient conditions, increased accumulation of H 2 O 2 is partially responsible for the root growth inhibition and NRAMP 2 is involved in remobilization of Mn in Golgi for root growth.