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Characterization of microRNAs from Arabidopsis galls highlights a role for miR159 in the plant response to the root‐knot nematode Meloidogyne incognita
Author(s) -
Medina Clémence,
Rocha Martine,
Magliano Marc,
Ratpopoulo Alizée,
Revel Benoît,
Marteu Nathalie,
Mag Virginie,
Lebrigand Kevin,
Cabrera Javier,
Barcala Marta,
Silva Ana Cláudia,
Millar Anthony,
Escobar Carolina,
Abad Pierre,
Favery Bruno,
JaubertPossamai Stéphanie
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14717
Subject(s) - biology , meloidogyne incognita , gall , arabidopsis , root knot nematode , terra incognita , nematode , myb , gene , nematode infection , rna , small rna , mutant , botany , gene expression , genetics , ecology
Summary Root knot nematodes ( RKN ) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. Micro RNA s (miRNAs) regulate gene expression during development and plant responses to various stresses. Disruption of post‐transcriptional gene silencing in Arabidopsis ago1 or ago2 mutants decrease the infection rate of RKN suggesting a role for this mechanism in the plant‐nematode interaction. By sequencing small RNA s from uninfected Arabidopsis roots and from galls 7 and 14 d post infection with Meloidogyne incognita , we identified 24 mi RNA s differentially expressed in gall as putative regulators of gall development. Moreover, strong activity within galls was detected for five mi RNA promoters. Analyses of nematode development in an Arabidopsis miR159abc mutant had a lower susceptibility to RKN , suggesting a role for the miR159 family in the plant response to M. incognita . Localization of mature miR159 within the giant and surrounding cells suggested a role in giant cell and gall. Finally, overexpression of miR159 in galls at 14 d post inoculation was associated with the repression of the miR159 target MYB 33 which expression is restricted to the early stages of infection. Overall, these results implicate the miR159 in plant responses to RKN .