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The mitochondrial pentatricopeptide repeat protein PPR 19 is involved in the stabilization of NADH dehydrogenase 1 transcripts and is crucial for mitochondrial function and Arabidopsis thaliana development
Author(s) -
Lee Kwanuk,
Han Ji Hoon,
Park YounIl,
Colas des FrancsSmall Catherine,
Small Ian,
Kang Hunseung
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14528
Subject(s) - pentatricopeptide repeat , biology , rna splicing , arabidopsis thaliana , arabidopsis , mitochondrion , gene , alternative splicing , genetics , trans splicing , rna binding protein , exon , microbiology and biotechnology , mutant , rna
Summary Despite the importance of pentatricopeptide repeat ( PPR ) proteins in organellar RNA metabolism and plant development, the functions of many PPR proteins remain unknown. Here, we determined the role of a mitochondrial PPR protein (At1g52620) comprising 19 PPR motifs, thus named PPR 19, in Arabidopsis thaliana . The ppr19 mutant displayed abnormal seed development, reduced seed yield, delayed seed germination, and retarded growth, indicating that PPR 19 is indispensable for normal growth and development of Arabidopsis thaliana . Splicing pattern analysis of mitochondrial genes revealed that PPR 19 specifically binds to the specific sequence in the 3′‐terminus of the NADH dehydrogenase 1 ( nad1 ) transcript and stabilizes transcripts containing the second and third exons of nad1 . Loss of these transcripts in ppr19 leads to multiple secondary effects on accumulation and splicing of other nad1 transcripts, from which we can infer the order in which cis ‐ and trans ‐spliced nad1 transcripts are normally processed. Improper splicing of nad1 transcripts leads to the absence of mitochondrial complex I and alteration of the nuclear transcriptome, notably influencing the alternative splicing of a variety of nuclear genes. Our results indicate that the mitochondrial PPR 19 is an essential component in the splicing of nad1 transcripts, which is crucial for mitochondrial function and plant development.