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Dynamic location changes of Bub1‐phosphorylated‐H2AThr133 with CENH3 nucleosome in maize centromeric regions
Author(s) -
Su Handong,
Liu Yalin,
Dong Qianhua,
Feng Chao,
Zhang Jing,
Liu Yang,
Birchler James A.,
Han Fangpu
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14415
Subject(s) - centromere , sister chromatids , biology , kinetochore , cohesin , chromosome segregation , microbiology and biotechnology , genetics , meiosis , chromosome , spindle checkpoint , bub1 , gene
Summary The genomic stability of all organisms requires precise cell division with proper chromosome orientation. The Bub1‐H2Aph‐Sgo1 pathway and spindle assembly checkpoint ( SAC ) components have been identified in yeast and mammals that are important for sister centromere orientation and chromosome segregation. However, their roles in plants are not clear. Maize meiotic mutants and minichromosomes were used to study the role of H2 AT hr133 phosphorylation and SAC components in sister centromere orientation and chromosome segregation. Unlike previously reported, SAC protein Bub1‐Sgo1 recruitment was independent of Rec8 in maize and did not play a role in centromere protection in meiosis I. Chromatin immunoprecipitation sequencing analysis with immnolocalization results indicate most CENH 3 nucleosomes contain phosphorylated H2 AT hr133 in centromeric regions. H2 AT hr133ph spreads to encompass centromeric regions including the inner centromeric and pericentromeric regions during (pro)metaphase. The presence and localization of SAC components and H2 AT hr133ph on maize lines containing sister chromatids separate precociously in anaphase I revealed no direct role of these proteins on centromere orientation in meiosis I . This work sheds light on the relationship between H2 AT hr133ph and CENH 3 nucleosome in plants, and the phosphorylation with dynamic location changes in centomeric regions suggests temporal and spatial regulation roles for H2A phosphorylation in chromosome segregation.