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The carnivorous Venus flytrap uses prey‐derived amino acid carbon to fuel respiration
Author(s) -
Fasbender Lukas,
Maurer Daniel,
Kreuzwieser Jürgen,
Kreuzer Ines,
Schulze Waltraud X.,
Kruse Jörg,
Becker Dirk,
Alfarraj Saleh,
Hedrich Rainer,
Werner Christiane,
Rennenberg Heinz
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14404
Subject(s) - metabolome , respiration , chemistry , metabolism , amino acid , glutamine , carnivorous plant , biochemistry , transcriptome , biology , environmental chemistry , botany , predation , gene expression , ecology , metabolite , gene
Summary The present study was performed to elucidate the fate of carbon (C) and nitrogen (N) derived from protein of prey caught by carnivorous Dionaea muscipula . For this, traps were fed 13 C/ 15 N‐glutamine (Gln). The release of 13 CO 2 was continuously monitored by isotope ratio infrared spectrometry. After 46 h, the allocation of C and N label into different organs was determined and tissues were subjected to metabolome, proteome and transcriptome analyses. Nitrogen of Gln fed was already separated from its C skeleton in the decomposing fluid secreted by the traps. Most of the Gln‐C and Gln‐N recovered inside plants were localized in fed traps. Among nonfed organs, traps were a stronger sink for Gln‐C compared to Gln‐N, and roots were a stronger sink for Gln‐N compared to Gln‐C. A significant amount of the Gln‐C was respired as indicated by 13 C‐ CO 2 emission, enhanced levels of metabolites of respiratory Gln degradation and increased abundance of proteins of respiratory processes. Transcription analyses revealed constitutive expression of enzymes involved in Gln metabolism in traps. It appears that prey not only provides building blocks of cellular constituents of carnivorous Dionaea muscipula , but also is used for energy generation by respiratory amino acid degradation.