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Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice
Author(s) -
He Yuqing,
Zhang Hehong,
Sun Zongtao,
Li Junmin,
Hong Gaojie,
Zhu Qisong,
Zhou Xuebiao,
MacFarlane Stuart,
Yan Fei,
Chen Jianping
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14376
Subject(s) - jasmonic acid , biology , brassinosteroid , immune system , virus , genetically modified rice , plant defense against herbivory , methyl jasmonate , innate immune system , host (biology) , virology , gene , mutant , botany , microbiology and biotechnology , genetics , transgene , genetically modified crops , arabidopsis
Summary Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone‐mediated immunity in dicot plants, little information is available on monocot–virus defense systems. We used a high‐throughput‐sequencing approach to compare the global gene expression of Rice black‐streaked dwarf virus ( RBSDV )‐infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid ( JA ) pathway was induced while the brassinosteroid ( BR ) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole ( BRZ ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide ( BL ) treatment increased RBSDV infection. Infection studies using coi1‐13 and Go mutants demonstrated JA ‐mediated resistance and BR ‐mediated susceptibility to RBSDV infection. A mixture of Me JA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. Me JA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor Os COI 1 . Collectively, our results reveal that JA ‐mediated defense can suppress the BR ‐mediated susceptibility to RBSDV infection.