Premium
Extensive translational regulation during seed germination revealed by polysomal profiling
Author(s) -
Bai Bing,
Peviani Alessia,
Horst Sjors,
Gamm Magdalena,
Snel Berend,
Bentsink Leónie,
Hanson Johannes
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14355
Subject(s) - germination , polysome , biology , arabidopsis , messenger rna , translational regulation , open reading frame , translation (biology) , translational efficiency , gene expression , microbiology and biotechnology , gene , genetics , botany , mutant , rna , peptide sequence , ribosome
Summary This work investigates the extent of translational regulation during seed germination. The polysome occupancy of each gene is determined by genome‐wide profiling of total mRNA and polysome‐associated mRNA . This reveals extensive translational regulation during Arabidopsis thaliana seed germination. The polysome occupancy of thousands of individual mRNA s changes to a large extent during the germination process. Intriguingly, these changes are restricted to two temporal phases (shifts) during germination, seed hydration and germination. Sequence features, such as upstream open reading frame number, transcript length, mRNA stability, secondary structures, and the presence and location of specific motifs correlated with this translational regulation. These features differed significantly between the two shifts, indicating that independent mechanisms regulate translation during seed germination. This study reveals substantial translational dynamics during seed germination and identifies development‐dependent sequence features and cis elements that correlate with the translation control, uncovering a novel and important layer of gene regulation during seed germination.