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Sl‐IAA27 regulates strigolactone biosynthesis and mycorrhization in tomato (var. MicroTom )
Author(s) -
Guillotin Bruno,
Etemadi Mohammad,
Audran Corinne,
Bouzayen Mondher,
Bécard Guillaume,
Combier JeanPhilippe
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14246
Subject(s) - strigolactone , biology , auxin , gene silencing , colonization , rna interference , gene , symbiosis , botany , microbiology and biotechnology , arabidopsis , genetics , mutant , rna , bacteria
Summary Root colonization by arbuscular mycorrhizal ( AM ) fungi is a complex and finely tuned process. Previous studies have shown that, among other plant hormones, auxin plays a role in this process but the specific involvement of Aux/ IAA s, the key regulators of auxin responses, is still unknown. In this study, we addressed the role of the tomato Sl‐IAA27 during AM symbiosis by using Sl‐IAA27‐RNAi and pSL‐IAA27 :: GUS stable tomato lines. The data show that Sl‐IAA27 expression is up‐regulated by the AM fungus and that silencing of Sl‐IAA27 has a negative impact on AM colonization. Sl‐IAA27 ‐silencing resulted in down‐regulation of three genes involved in strigolactone synthesis, NSP1, D27 and MAX1 , and treatment of Sl‐IAA27 ‐silenced plants with the strigolactone analog GR24 complemented their mycorrhizal defect phenotype. Overall, the study identified an Aux/ IAA gene as a new component of the signaling pathway controlling AM fungal colonization in tomato. This gene is proposed to control strigolactone biosynthesis via the regulation of NSP1 .