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The class III peroxidase PRX 17 is a direct target of the MADS ‐box transcription factor AGAMOUS‐LIKE15 ( AGL 15) and participates in lignified tissue formation
Author(s) -
Cosio Claudia,
Ranocha Philippe,
Francoz Edith,
Burlat Vincent,
Zheng Yumei,
Perry Sharyn E.,
Ripoll JuanJose,
Yanofsky Martin,
Dunand Christophe
Publication year - 2017
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.14127
Subject(s) - agamous , mads box , transcription factor , microbiology and biotechnology , biology , arabidopsis thaliana , chromatin immunoprecipitation , nicotiana benthamiana , arabidopsis , biochemistry , mutant , gene expression , gene , promoter
Summary Several physiological functions have been attributed to class III peroxidases (PRXs) in plants, but the in planta role of most members of this family still remains undetermined. Here, we report the first functional characterization of PRX 17 (At2g22420), one of the 73 members of this family in Arabidopsis thaliana . Localization of PRX 17 was examined by transient expression in Nicotiana benthamiana . Loss‐ and gain‐of‐function mutants in A. thaliana were studied. Regulation at the gene and protein levels was analyzed using β‐glucuronidase ( GUS ) activity, quantitative reverse transcriptase ( qRT )‐ PCR , zymography, and chromatin immunoprecipitation. Phenotypes were characterized including lignin and xylan contents. PRX 17 was expressed in various tissues, including vascular tissues, and PRX 17 was localized to the cell wall. In prx17 , the lignin content was reduced in the stem and siliques and bolting was delayed, while the opposite phenotype was observed in 35S : PRX 17 plants, together with a significant increase of lignin and xylan immunofluorescence signal. Finally, we demonstrated that the transcription factor AGAMOUS‐LIKE15 ( AGL 15) binds to the PRX 17 promoter and regulates PRX 17 expression level. This converging set of structural, transcriptomic and physiological data suggests that PRX 17, under the control of AGL 15, contributes to developmental programs by playing an essential role in regulating age‐dependent lignified tissue formation, including changes in cell wall properties.