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Retrograde trafficking from the endosome to the trans‐Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum
Author(s) -
Zheng Wenhui,
Zheng Huawei,
Zhao Xu,
Zhang Ying,
Xie Qiurong,
Lin Xiaolian,
Chen Ahai,
Yu Wenying,
Lu Guodong,
Shim WonBo,
Zhou Jie,
Wang Zonghua
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13867
Subject(s) - retromer , endosome , microbiology and biotechnology , biology , vacuolar protein sorting , transport protein , golgi apparatus , conidiation , escrt , mutant , genetics , intracellular , gene , endoplasmic reticulum
Summary In eukaryotes, the retromer is an endosome‐localized complex involved in protein retrograde transport. However, the role of such intracellular trafficking events in pathogenic fungal development and pathogenicity remains unclear. The role of the retromer complex in Fusarium graminearum was investigated using cell biological and genetic methods. We observed the retromer core component FgVps35 (Vacuolar Protein Sorting 35) in the cytoplasm as fast‐moving puncta. FgVps35‐ GFP co‐localized with both early and late endosomes, and associated with the trans‐Golgi network ( TGN ), suggesting that FgVps35 functions at the donor endosome membrane to mediate TGN trafficking. Disruption of microtubules with nocodazole significantly restricted the transportation of FgVps35‐ GFP and resulted in severe germination and growth defects. Mutation of Fg VPS 35 not only mimicked growth defects induced by pharmacological treatment, but also affected conidiation, ascospore formation and pathogenicity. Using yeast two‐hybrid assays, we determined the interactions among FgVps35, FgVps26, FgVps29, FgVps17 and FgVps5 which are analogous to the yeast retromer complex components. Deletion of any one of these genes resulted in similar phenotypic defects to those of the ΔFgvps35 mutant and disrupted the stability of the complex. Overall, our results provide the first clear evidence of linkage between the retrograde transport mediated by the retromer complex and virulence in F. graminearum .