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The rice DUF 1620‐containing and WD 40‐like repeat protein is required for the assembly of the restoration of fertility complex
Author(s) -
Qin Xiaojian,
Huang Qi,
Xiao Haijun,
Zhang Qiannan,
Ni Chenzi,
Xu Yanghong,
Liu Gai,
Yang Daichang,
Zhu Yingguo,
Hu Jun
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13824
Subject(s) - biology , rna interference , cytoplasmic male sterility , microbiology and biotechnology , cytoplasm , protein subunit , genetics , gene , rna
Summary Cytoplasmic male sterility ( CMS ) and restoration of fertility ( Rf ) are widely distributed in plant species utilized by humans. RF 5 and GRP 162 are subunits of the restoration of fertility complex ( RFC ) in Hong‐Lian rice. Despite the fact that the RFC is 400–500 kDa in size, the other proteins or factors in the complex still remain unknown. Here, we identified RFC subunit 3, which encodes a DUF 1620‐containing and WD 40‐like repeat protein ( RFC 3) that is present in all tissues but highly expressed in leaves. We established that RFC 3 interacts with both RF 5 and GRP 162 in vitro and in vivo , and is transported into the mitochondria as a membrane protein. Furthermore, CMS RNA ( atp6‐orfH79 ) and CMS cytotoxic protein ( ORFH 79) accumulate when RFC 3 is silenced in restorer lines. We presented the analysis with blue‐native polyacrylamide gel electrophoresis, indicating that RFC is disrupted in the RNA i line. We concluded that RCF 3 is indispensable as a scaffold protein for the assembly of the RFC complex. We unveil a new molecular player of the RFC in the Rf pathway in rice and propose the model of RFC based on these data.