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An antibody that confers plant disease resistance targets a membrane‐bound glyoxal oxidase in Fusarium
Author(s) -
Song XiuShi,
Xing Shu,
Li HePing,
Zhang JingBo,
Qu Bo,
Jiang JinHe,
Fan Chao,
Yang Peng,
Liu JinLong,
Hu ZuQuan,
Xue Sheng,
Liao YuCai
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13806
Subject(s) - plant disease resistance , antibody , biochemistry , oxidase test , plant disease , microbiology and biotechnology , chemistry , fusarium , biology , plant immunity , enzyme , botany , genetics , gene , arabidopsis , mutant
Summary Plant germplasm resources with natural resistance against globally important toxigenic Fusarium are inadequate. CWP 2, a Fusarium genus‐specific antibody, confers durable resistance to different Fusarium pathogens that infect cereals and other crops, producing mycotoxins. However, the nature of the CWP 2 target is not known. Thus, investigation of the gene coding for the CWP 2 antibody target will likely provide critical insights into the mechanism underlying the resistance mediated by this disease‐resistance antibody. Immunoblots and mass spectrometry analysis of two‐dimensional electrophoresis gels containing cell wall proteins from Fusarium graminearum ( Fg ) revealed that a glyoxal oxidase ( GLX ) is the CWP 2 antigen. Cellular localization studies showed that GLX is localized to the plasma membrane. This GLX efficiently catalyzes hydrogen peroxide production; this enzymatic activity was specifically inhibited by the CWP 2 antibody. GLX ‐deletion strains of Fg , F. verticillioides ( Fv ) and F. oxysporum had significantly reduced virulence on plants. The GLX ‐deletion Fg and Fv strains had markedly reduced mycotoxin accumulation, and the expression of key genes in mycotoxin metabolism was downregulated. This study reveals a single gene‐encoded and highly conserved cellular surface antigen that is specifically recognized by the disease‐resistance antibody CWP 2 and regulates both virulence and mycotoxin biosynthesis in Fusarium species.

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