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The Craterostigma plantagineum glycine‐rich protein Cp GRP 1 interacts with a cell wall‐associated protein kinase 1 (Cp WAK 1) and accumulates in leaf cell walls during dehydration
Author(s) -
Giarola Valentino,
Krey Stephanie,
Driesch Barbara,
Bartels Dorothea
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13766
Subject(s) - apoplast , cell wall , abscisic acid , dehydration , microbiology and biotechnology , biology , protein kinase a , kinase , gene , gene expression , biochemistry , desiccation tolerance , desiccation , botany
SummaryCraterostigma plantagineum tolerates extreme desiccation. Leaves of this plant shrink and extensively fold during dehydration and expand again during rehydration, preserving their structural integrity. Genes were analysed that may participate in the reversible folding mechanism. Analysis of transcripts abundantly expressed in desiccated leaves identified a gene putatively coding for an apoplastic glycine‐rich protein (CpGRP1). We studied the expression, regulation and subcellular localization of CpGRP1 and its ability to interact with a cell wall‐associated protein kinase (CpWAK1) to understand the role of CpGRP1 in the cell wall during dehydration. The CpGRP1 protein accumulates in the apoplast of desiccated leaves. Analysis of the promoter revealed that the gene expression is mainly regulated at the transcriptional level, is independent of abscisic acid (ABA) and involves a drought‐responsive cis ‐element (DRE). CpGRP1 interacts with CpWAK1 which is down‐regulated in response to dehydration. Our data suggest a role of the CpGRP1–CpWAK1 complex in dehydration‐induced morphological changes in the cell wall during dehydration in C. plantagineum . Cell wall pectins and dehydration‐induced pectin modifications are predicted to be involved in the activity of the CpGRP1–CpWAK1 complex.