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Nitrate reductase mutation alters potassium nutrition as well as nitric oxide‐mediated control of guard cell ion channels in Arabidopsis
Author(s) -
Chen ZhongHua,
Wang Yizhou,
Wang JianWen,
Babla Mohammad,
Zhao Chenchen,
GarcíaMata Carlos,
Sani Emanuela,
Differ Christopher,
Mak Michelle,
Hills Adrian,
Amtmann Anna,
Blatt Michael R.
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13714
Subject(s) - guard cell , arabidopsis , potassium channel , nitrate reductase , mutant , microbiology and biotechnology , inward rectifier potassium ion channel , chemistry , nitric oxide , ion channel , biochemistry , biology , biophysics , gene , enzyme , receptor , organic chemistry
Summary Maintaining potassium (K + ) nutrition and a robust guard cell K + inward channel activity is considered critical for plants’ adaptation to fluctuating and challenging growth environment. ABA induces stomatal closure through hydrogen peroxide and nitric oxide ( NO ) along with subsequent ion channel‐mediated loss of K + and anions. However, the interactions of NO synthesis and signalling with K + nutrition and guard cell K + channel activities have not been fully explored in Arabidopsis . Physiological and molecular techniques were employed to dissect the interaction of nitrogen and potassium nutrition in regulating stomatal opening, CO 2 assimilation and ion channel activity. These data, gene expression and ABA signalling transduction were compared in wild‐type Columbia‐0 (Col‐0) and the nitrate reductase mutant nia1nia2 . Growth and K + nutrition were impaired along with stomatal behaviour, membrane transport, and expression of genes associated with ABA signalling in the nia1nia2 mutant. ABA ‐inhibited K + in current and ABA ‐enhanced slow anion current were absent in nia1nia2 . Exogenous NO restored regulation of these channels for complete stomatal closure in nia1nia2 . While NO is an important signalling component in ABA ‐induced stomatal closure in Arabidopsis , our findings demonstrate a more complex interaction associating potassium nutrition and nitrogen metabolism in the nia1nia2 mutant that affects stomatal function.