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Monoterpene biosynthesis potential of plant subcellular compartments
Author(s) -
Dong Lemeng,
Jongedijk Esmer,
Bouwmeester Harro,
Van Der Krol Alexander
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13629
Subject(s) - plastid , cytosol , mitochondrion , biochemistry , atp synthase , biosynthesis , geraniol , biology , chloroplast , botany , enzyme , gene , essential oil
Summary Subcellular monoterpene biosynthesis capacity based on local geranyl diphosphate ( GDP ) availability or locally boosted GDP production was determined for plastids, cytosol and mitochondria. A geraniol synthase ( GES ) was targeted to plastids, cytosol, or mitochondria. Transient expression in Nicotiana benthamiana indicated local GDP availability for each compartment but resulted in different product levels. A GDP synthase from Picea abies ( Pa GDPS 1 ) was shown to boost GDP production. Pa GDPS 1 was also targeted to plastids, cytosol or mitochondria and Pa GDPS 1 and GES were coexpressed in all possible combinations. Geraniol and geraniol‐derived products were analyzed by GC ‐ MS and LC ‐ MS , respectively. GES product levels were highest for plastid‐targeted GES , followed by mitochondrial‐ and then cytosolic‐targeted GES . For each compartment local boosting of GDP biosynthesis increased GES product levels. GDP exchange between compartments is not equal: while no GDP is exchanged from the cytosol to the plastids, 100% of GDP in mitochondria can be exchanged to plastids, while only 7% of GDP from plastids is available for mitochondria. This suggests a direct exchange mechanism for GDP between plastids and mitochondria. Cytosolic Pa GDPS 1 competes with plastidial GES activity, suggesting an effective drain of isopentenyl diphosphate from the plastids to the cytosol.