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Guard cell hydrogen peroxide and nitric oxide mediate elevated CO 2 ‐induced stomatal movement in tomato
Author(s) -
Shi Kai,
Li Xin,
Zhang Huan,
Zhang Guanqun,
Liu Yaru,
Zhou Yanhong,
Xia Xiaojian,
Chen Zhixiang,
Yu Jingquan
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13621
Subject(s) - guard cell , abscisic acid , nitric oxide , microbiology and biotechnology , chemistry , hydrogen peroxide , stomatal conductance , gene silencing , transpiration , nitrate reductase , biology , botany , photosynthesis , biochemistry , gene , enzyme , organic chemistry
Summary Climate change as a consequence of increasing atmospheric CO 2 influences plant photosynthesis and transpiration. Although the involvement of stomata in plant responses to elevated CO 2 has been well established, the underlying mechanism of elevated CO 2 ‐induced stomatal movement remains largely unknown. We used diverse techniques, including laser scanning confocal microscopy, transmission electron microscopy, biochemical methodologies and gene silencing to investigate the signaling pathway for elevated CO 2 ‐induced stomatal movement in tomato ( Solanum lycopersicum ). Elevated CO 2 ‐induced stomatal closure was dependent on the production of RESPIRATORY BURST OXIDASE 1 ( RBOH 1 )‐mediated hydrogen peroxide (H 2 O 2 ) and NITRATE REDUCTASE ( NR )‐mediated nitric oxide ( NO ) in guard cells in an abscisic acid ( ABA )‐independent manner. Silencing of OPEN STOMATA 1 ( OST 1 ) compromised the elevated CO 2 ‐induced accumulation of H 2 O 2 and NO , upregulation of SLOW ANION CHANNEL ASSOCIATED 1 ( SLAC 1 ) gene expression and reduction of stomatal aperture, whereas silencing of RBOH 1 or NR had no effects on the expression of OST 1 . Our results demonstrate that as critical signaling molecules, RBOH 1 ‐dependent H 2 O 2 and NR ‐dependent NO act downstream of OST 1 that regulate SLAC 1 expression and elevated CO 2 ‐induced stomatal movement. This information is crucial to deepen the understanding of CO 2 signaling pathway in guard cells.