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Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom‐2 resistance gene in melon
Author(s) -
Schmidt Sarah Maria,
Lukasiewicz Joanna,
Farrer Rhys,
Dam Peter,
Bertoldo Chiara,
Rep Martijn
Publication year - 2016
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13584
Subject(s) - fusarium wilt , biology , fusarium oxysporum , melon , gene , plant disease resistance , genetics , genome , cucumis , botany , horticulture
Summary Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called ‘avirulence’ proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics‐based genome comparison and genetic transformation of the fungus to identify AVRFOM2 , the gene that encodes the avirulence protein recognized by the melon Fom‐2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom‐2 ‐harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi. The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom‐2 ‐containing cultivars in the field.