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Standards for plant synthetic biology: a common syntax for exchange of DNA parts
Author(s) -
Patron Nicola J.,
Orzaez Diego,
Marillonnet Sylvestre,
Warzecha Heribert,
Matthewman Colette,
Youles Mark,
Raitskin Oleg,
Leveau Aymeric,
Farré Gemma,
Rogers Christian,
Smith Alison,
Hibberd Julian,
Webb Alex A. R.,
Locke James,
Schornack Sebastian,
Ajioka Jim,
Baulcombe David C.,
Zipfel Cyril,
Kamoun Sophien,
Jones Jonathan D. G.,
Kuhn Hannah,
Robatzek Silke,
Van Esse H. Peter,
Sanders Dale,
Oldroyd Giles,
Martin Cathie,
Field Rob,
O'Connor Sarah,
Fox Samantha,
Wulff Brande,
Miller Ben,
Breakspear Andy,
Radhakrishnan Guru,
Delaux PierreMarc,
Loqué Dominique,
Granell Antonio,
Tissier Alain,
Shih Patrick,
Brutnell Thomas P.,
Quick W. Paul,
Rischer Heiko,
Fraser Paul D.,
Aharoni Asaph,
Raines Christine,
South Paul F.,
Ané JeanMichel,
Hamberger Björn R.,
Langdale Jane,
Stougaard Jens,
Bouwmeester Harro,
Udvardi Michael,
Murray James A. H.,
Ntoukakis Vardis,
Schäfer Patrick,
Denby Katherine,
Edwards Keith J.,
Osbourn Anne,
Haseloff Jim
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13532
Subject(s) - synthetic biology , syntax , plant biology , dna , biology , chemistry , botany , computational biology , computer science , genetics , artificial intelligence
Summary Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease‐mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.