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Characterization of an inducible C 2 H 2 ‐type zinc finger transcription factor Vu STOP 1 in rice bean ( Vigna umbellata ) reveals differential regulation between low pH and aluminum tolerance mechanisms
Author(s) -
Fan Wei,
Lou He Qiang,
Gong Yu Long,
Liu Mei Ya,
Cao Meng Jie,
Liu Yu,
Yang Jian Li,
Zheng Shao Jian
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13456
Subject(s) - biology , transactivation , bimolecular fluorescence complementation , zinc finger , complementation , transcription factor , vigna , gene expression , zinc finger transcription factor , gene , regulation of gene expression , protein fragment complementation assay , repressor , microbiology and biotechnology , genetics , botany , mutant
Summary The rice bean ( Vigna umbellata ) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 ( Vu MATE 1 ) under aluminum (Al 3+ ) stress. However, the underlying mechanisms regulating Vu MATE 1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 ( STOP 1)‐like protein, Vu STOP 1 , from rice bean. The role of Vu STOP 1 in regulating Vu MATE 1 expression was investigated using the yeast one‐hybrid assay. We characterized the function of Vu STOP 1 in Al 3 + ‐ and H + ‐tolerance using in planta complementation assays. We demonstrated that Vu STOP 1 has transactivation potential. We found that Vu STOP 1 expression is inducible by Al 3+ and H + stress. However, although Vu STOP 1 binds to the promoter of Vu MATE 1 , the inconsistent tissue localization patterns of Vu STOP 1 and Vu MATE 1 preclude Vu STOP 1 as the major factor regulating Vu MATE 1 expression. In addition, when a protein translation inhibitor increased expression of Vu STOP 1 , Vu MATE 1 expression was inhibited. In planta complementation assay demonstrated that Vu STOP 1 could fully restore expression of genes involved in H + tolerance, but could only partially restore expression of At MATE . We conclude that Vu STOP 1 plays a major role in H + tolerance, but only a minor role in Al 3+ tolerance. The differential transcriptional regulation of Vu STOP 1 and Vu MATE 1 reveals a complex regulatory system controlling Vu MATE 1 expression.