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Integration of genetic and physical maps of the P rimula vulgaris S locus and localization by chromosome in situ hybridization
Author(s) -
Li Jinhong,
Webster Margaret A.,
Wright Jonathan,
Cocker Jonathan M.,
Smith Matthew C.,
Badakshi Farah,
HeslopHarrison Pat,
Gilmartin Philip M.
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13373
Subject(s) - locus (genetics) , physical mapping , genetics , in situ hybridization , biology , in situ , gene mapping , chromosome , gene , microbiology and biotechnology , gene expression , chemistry , organic chemistry
Summary Heteromorphic flower development in P rimula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S ‐linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three‐point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5‐Mb genomic region with 1 Mb of sequence containing 82 S ‐linked genes anchored to overlapping BAC s. The S locus is located close to the centromere of the largest metacentric chromosome pair. These data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus.