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RNA seq reveals weed‐induced PIF 3‐like as a candidate target to manipulate weed stress response in soybean
Author(s) -
Horvath David P.,
Hansen Stephanie A.,
MorilesMiller Janet P.,
Pierik Ronald,
Yan Changhui,
Clay David E.,
Scheffler Brian,
Clay Sharon A.
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13351
Subject(s) - weed , jasmonic acid , biology , gene , transcriptome , gene expression , agronomy , botany , genetics
Summary Weeds reduce yield in soybeans ( Glycine max ) through incompletely defined mechanisms. The effects of weeds on the soybean transcriptome were evaluated in field conditions during four separate growing seasons. RNAS eq data were collected from six biological samples of soybeans growing with or without weeds. Weed species and the methods to maintain weed‐free controls varied between years to mitigate treatment effects, and to allow detection of general soybean weed responses. Soybean plants were not visibly nutrient‐ or water‐stressed. We identified 55 consistently downregulated genes in weedy plots. Many of the downregulated genes were heat shock genes. Fourteen genes were consistently upregulated. Several transcription factors including a PHYTOCHROME INTERACTING FACTOR 3 ‐like gene ( PIF 3 ) were included among the upregulated genes. Gene set enrichment analysis indicated roles for increased oxidative stress and jasmonic acid signaling responses during weed stress. The relationship of this weed‐induced PIF 3 gene to genes involved in shade avoidance responses in Arabidopsis provide evidence that this gene may be important in the response of soybean to weeds. These results suggest that the weed‐induced PIF 3 gene will be a target for manipulating weed tolerance in soybean.