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The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)‐β‐glucan levels and HvCslF gene transcript abundance
Author(s) -
Aditya Jessika,
Lewis John,
Shirley Neil J.,
Tan HweiTing,
Henderson Marilyn,
Fincher Geoffrey B.,
Burton Rachel A.,
Mather Diane E.,
Tucker Matthew R.
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13349
Subject(s) - biology , heterodera avenae , hordeum vulgare , cultivar , hordeum , nematode infection , cell wall , gene , glucan , nematode , poaceae , botany , microbiology and biotechnology , biochemistry , ecology
SummaryHeterodera avenae (cereal cyst nematode, CCN ) infects the roots of barley ( Hordeum vulgare ) forming syncytial feeding sites. In resistant host plants, relatively few females develop to maturity. Little is known about the physiological and biochemical changes induced during CCN infection. Responses to CCN infection were investigated in resistant ( Rha2 ) and susceptible barley cultivars through histological, compositional and transcriptional analysis. Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation. Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)‐β‐glucan. Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE ‐ LIKE F10 ( HvCslF10 ), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars. In barley, Rha2 ‐mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall‐related transcript abundance and changes in cell wall composition. During H . avenae infection, (1,3;1,4)‐β‐glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)‐β‐glucan during dicot cyst nematode infections. Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)‐β‐glucan synthesis, suggest a role for these genes in the CCN infection process.