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Natural variation for anthocyanin accumulation under high‐light and low‐temperature stress is attributable to the ENHANCER OF AG ‐4 2 ( HUA 2 ) locus in combination with PRODUCTION OF ANTHOCYANIN PIGMENT 1 ( PAP 1 ) and PAP 2
Author(s) -
Ilk Nadine,
Ding Jia,
Ihnatowicz Anna,
Koornneef Maarten,
Reymond Matthieu
Publication year - 2015
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13177
Subject(s) - anthocyanin , quantitative trait locus , biology , genetics , complementation , locus (genetics) , mutant , gene , arabidopsis thaliana , botany
Summary Growing conditions combining high light intensities and low temperatures lead to anthocyanin accumulation in plants. This response was contrasted between two A rabidopsis thaliana accessions, which were used to decipher the genetic and molecular bases underlying the variation of this response. Quantitative trait loci ( QTL s) for flowering time ( FT ) and anthocyanin accumulation under a high‐light and low‐temperature scenario versus a control environment were mapped. Major QTL s were confirmed using near‐isogenic lines. Candidate genes were examined using mutants and gene expression studies as well as transgenic complementation. Several QTL s were found for FT and for anthocyanin content, of which one QTL co‐located at the ENHANCER OF AG ‐4 2 ( HUA 2 ) locus. That HUA 2 is a regulator of both pathways was confirmed by the analysis of loss‐of‐function mutants. For a strong expression of anthocyanin, additional allelic variation was detected for the PRODUCTION OF ANTHOCYANIN PIGMENT 1 ( PAP 1 ) and PAP 2 genes which control the anthocyanin pathway. The genetic control of variation for anthocyanin content was dissected in A . thaliana and shown to be affected by a common regulator of flowering and anthocyanin biosynthesis together with anthocyanin‐specific regulators.