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Irrepressible MONOPTEROS / ARF 5 promotes de novo shoot formation
Author(s) -
Ckurshumova Wenzislava,
Smirnova Tatiana,
Marcos Danielle,
Zayed Yara,
Berleth Thomas
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13014
Subject(s) - shoot , cytokinin , auxin , biology , regeneration (biology) , plant hormone , microbiology and biotechnology , arabidopsis , callus , botany , biochemistry , gene , mutant
SummaryIn vitro regeneration of complete organisms from diverse cell types is a spectacular property of plant cells. Despite the great importance of plant regeneration for plant breeding and biotechnology, its molecular basis is still largely unclear and many important crop plants have remained recalcitrant to regeneration. Hormone‐exposure protocols to trigger the de novo formation of either roots or shoots from callus tissue demonstrate the importance of auxin and cytokinin signaling pathways, and genetic differences in these pathways may contribute to the highly divergent responsiveness of plant species to regeneration protocols. In this study, we show that signaling through MONOPTEROS ( MP )/ AUXIN RESPONSE FACTOR 5 is necessary for the formation of shoots from A rabidopsis calli. Most strikingly, an irrepressible variant of MP , MP Δ , is sufficient for promoting de novo shoot formation through pathways involving the genetically downstream functions of SHOOT MERISTEMLESS ( STM ) and CYTOKININ RESPONSE FACTOR 2 ( CRF 2 ). We conclude that the MP Δ genotype can promote de novo shoot formation and can be used to probe corresponding signaling pathways.