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Translatome profiling in dormant and nondormant sunflower ( Helianthus annuus ) seeds highlights post‐transcriptional regulation of germination
Author(s) -
Layat Elodie,
Leymarie Juliette,
ElMaaroufBouteau Hayat,
Caius José,
Langlade Nicolas,
Bailly Christophe
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.13002
Subject(s) - imbibition , germination , biology , dormancy , polysome , helianthus annuus , microbiology and biotechnology , botany , embryo , sunflower , seed dormancy , abscisic acid , rna , genetics , horticulture , gene , ribosome
Summary Seed dormancy, which blocks germination in apparently favourable conditions, is a key regulatory control point of plant population establishment. As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome‐associated mRNA s, that is, the translatome, were fractionated and characterized with microarrays in dormant and nondormant sunflower ( H elianthus annuus ) embryos during their imbibition at 10°C, a temperature preventing germination of dormant embryos. Profiling of mRNA s in polysomal complexes revealed that the translatome differs between germinating and nongerminating embryos. Association of transcripts with polysomes reached a maximum after 15 h of imbibition; at this time‐point 194 polysome‐associated transcripts were specifically found in nondormant embryos and 47 in dormant embryos only. The proteins corresponding to the polysomal mRNA s in nondormant embryos appeared to be very pertinent for germination and were involved mainly in transport, regulation of transcription or cell wall modifications. This work demonstrates that seed germination results from a timely regulated and selective recruitment of mRNA s to polysomes, thus opening novel fields of investigation for the understanding of this developmental process.