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Involvement of two‐component signalling systems in the regulation of stomatal aperture by light in Arabidopsis thaliana
Author(s) -
Marchadier Elodie,
Hetherington Alistair M.
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12813
Subject(s) - guard cell , cytokinin , abscisic acid , arabidopsis thaliana , microbiology and biotechnology , arabidopsis , biology , histidine kinase , kinase , gtpase , signalling , two component regulatory system , histidine , biochemistry , gene , auxin , amino acid , mutant
Summary Two‐component signalling ( TCS ) systems play important roles in cytokinin and ethylene signalling in Arabidopsis thaliana . Although the involvement of histidine kinases ( AHK s) in drought stress responses has been described, their role and that of histidine phosphotransferases ( AHP s) in guard cell signalling remain to be fully elucidated. Here, we investigated the roles of TCS genes, the histidine phosphotransferase AHP 2 and the histidine kinases AHK 2 and AHK 3 , previously reported to play roles in cytokinin and abscisic acid ( ABA ) signalling. We show that AHP 2 is present in the nucleus and the cytoplasm, and is involved in light‐induced opening. We also present evidence that there is some redistribution of AHP 2 from the nucleus to the cytoplasm on addition of ABA . In addition, we provide data to support a role for the cytokinin receptors AHK 2 and AHK 3 in light‐induced stomatal opening and, by inference, in controlling the stomatal sensitivity to ABA . Our results provide new insights into the operation of TCS in plants, cross‐talk in stomatal signalling and, in particular, the process of light‐induced stomatal opening.