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Transcriptomic and lipidomic profiles of glycerolipids during A rabidopsis flower development
Author(s) -
Nakamura Yuki,
Teo Norman Z. W.,
Shui Guanghou,
Chua Christine H. L.,
Cheong WeiFun,
Parameswaran Sriram,
Koizumi Ryota,
Ohta Hiroyuki,
Wenk Markus R.,
Ito Toshiro
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12774
Subject(s) - arabidopsis , phosphatidic acid , biology , transcriptome , phosphatidylinositol , lipidome , galactolipid , microbiology and biotechnology , diacylglycerol kinase , biochemistry , gene expression profiling , galactolipids , lipid metabolism , botany , gene expression , mutant , gene , signal transduction , phospholipid , chloroplast , protein kinase c , membrane
Summary Flower glycerolipids are the yet‐to‐be discovered frontier of the lipidome. Although ample evidence suggests important roles for glycerolipids in flower development, stage‐specific lipid profiling in tiny A rabidopsis flowers is challenging. Here, we utilized a transgenic system to synchronize flower development in A rabidopsis. The transgenic plant PAP 1:: AP 1‐ GR ap1‐1 cal‐5 showed synchronized flower development upon dexamethasone treatment, which enabled massive harvesting of floral samples of homogenous developmental stages for glycerolipid profiling. Glycerolipid profiling revealed a decrease in concentrations of phospholipids involved in signaling during the early development stages, such as phosphatidic acid and phosphatidylinositol, and a marked increase in concentrations of nonphosphorous galactolipids during the late stage. Moreover, in the midstage, phosphatidylinositol 4,5‐bisphosphate concentration was increased transiently, which suggests the stimulation of the phosphoinositide metabolism. Accompanying transcriptomic profiling of relevant glycerolipid metabolic genes revealed simultaneous induction of multiple phosphoinositide biosynthetic genes associated with the increased phosphatidylinositol 4,5‐bisphosphate concentration, with a high degree of differential expression patterns for genes encoding other glycerolipid‐metabolic genes. The phosphatidic acid phosphatase mutant pah1 pah2 showed flower developmental defect, suggesting a role for phosphatidic acid in flower development. Our concurrent profiling of glycerolipids and relevant metabolic gene expression revealed distinct metabolic pathways stimulated at different stages of flower development in A rabidopsis.