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Nitrogen dioxide regulates organ growth by controlling cell proliferation and enlargement in A rabidopsis
Author(s) -
Takahashi Misa,
Furuhashi Takamasa,
Ishikawa Naoko,
Horiguchi Gorou,
Sakamoto Atsushi,
Tsukaya Hirokazu,
Morikawa Hiromichi
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12609
Subject(s) - cell growth , biology , nutrient , plant growth , sowing , botany , biochemistry , ecology
Summary To gain more insight into the physiological function of nitrogen dioxide ( NO 2 ), we investigated the effects of exogenous NO 2 on growth in A rabidopsis thaliana . Plants were grown in air without NO 2 for 1 wk after sowing and then grown for 1–4 wk in air with (designated treated plants) or without (control plants) NO 2 . Plants were irrigated semiweekly with a nutrient solution containing 19.7 mM nitrate and 10.3 mM ammonium. Five‐week‐old plants treated with 50 ppb NO 2 showed a ≤ 2.8‐fold increase in biomass relative to controls. Treated plants also showed early flowering. The magnitude of the effects of NO 2 on leaf expansion, cell proliferation and enlargement was greater in developing than in maturing leaves. Leaf areas were 1.3–8.4 times larger on treated plants than corresponding leaves on control plants. The NO 2 ‐induced increase in leaf size was largely attributable to cell proliferation in developing leaves, but was attributable to both cell proliferation and enlargement in maturing leaves. The expression of different sets of genes for cell proliferation and/or enlargement was induced by NO 2 , but depended on the leaf developmental stage. Collectively, these results indicated that NO 2 regulates organ growth by controlling cell proliferation and enlargement.