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A MYB coiled‐coil transcription factor interacts with NSP 2 and is involved in nodulation in L otus japonicus
Author(s) -
Kang Heng,
Chu Xiaojie,
Wang Chao,
Xiao Aifang,
Zhu Hui,
Yuan Songli,
Yang Zhenzhen,
Ke Danxia,
Xiao Shaobo,
Hong Zonglie,
Zhang Zhongming
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12593
Subject(s) - biology , myb , bimolecular fluorescence complementation , transcription factor , gene knockdown , microbiology and biotechnology , transcription (linguistics) , gene expression , gene , biochemistry , linguistics , philosophy
Summary Transcription factor complex formation is a central step in regulating gene expression. In this report, a novel MYB coiled‐coil transcription factor referred to as IPN 2, for I nteracting P rotein of NSP 2, is described. The interaction between IPN 2 and NSP 2 was examined by protein pull‐down assays and bimolecular fluorescence complementation ( B i FC ). Subcellular localization of proteins, gene expression and gene function were assessed in transgenic hairy roots expressing tagged recombinant proteins, promoter‐reporter and RNA interference ( RNA i) constructs, respectively. The GRAS domain of NSP 2 and the coiled‐coil domain of IPN 2 were found to be responsible for the interaction between the two proteins. IPN 2 had strong transcription activation activity, bound directly to the NIN gene promoter, and was localized to the nuclei of Lotus japonicus root cells. The expression of IPN 2 was elevated during nodule development, coinciding with increased NSP 2 gene expression during nodule organogenesis. RNA i‐mediated knockdown expression of IPN 2 did not affect arbuscular mycorrhizal development, but had deleterious effects on rhizobial infection and nodule formation in L . japonicus . These results demonstrate an important role of IPN 2 in nodule organogenesis and place a new MYB transcription factor in the N od signaling pathway.