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The grapevine flagellin receptor Vv FLS 2 differentially recognizes flagellin‐derived epitopes from the endophytic growth‐promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria
Author(s) -
Trdá Lucie,
Fernandez Olivier,
Boutrot Freddy,
Héloir MarieClaire,
Kelloniemi Jani,
Daire Xavier,
Adrian Marielle,
Clément Christophe,
Zipfel Cyril,
Dorey Stéphan,
Poinssot Benoit
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12592
Subject(s) - flagellin , biology , arabidopsis , arabidopsis thaliana , burkholderia cenocepacia , epitope , bacteria , pseudomonas syringae , microbiology and biotechnology , pattern recognition receptor , burkholderia , immune system , mutant , gene , innate immune system , genetics , antigen
Summary The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22– FLAGELLIN SENSING 2 ( FLS 2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth‐promoting rhizobacterium ( PGPR ) B urkholderia phytofirmans . The functionality of the grapevine FLS 2 receptor, Vv FLS 2, was demonstrated by complementation assays in the A rabidopsis thaliana fls2 mutant, which restored flg22‐induced H 2 O 2 production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between V v FLS 2 and At FLS 2. In grapevine, flg22‐triggered immune responses are conserved and led to partial resistance against B otrytis cinerea . Unlike flg22 peptides derived from P seudomonas aeruginosa or X anthomonas campestris , flg22 peptide derived from B . phytofirmans triggered only a small oxidative burst, weak and transient defense gene induction and no growth inhibition in grapevine. Although, in A rabidopsis, all the flg22 epitopes exhibited similar biological activities, the expression of V v FLS 2 into the fls2 background conferred differential flg22 responses characteristic for grapevine. These results demonstrate that V v FLS 2 differentially recognizes flg22 from different bacteria, and suggest that flagellin from the beneficial PGPR B . phytofirmans has evolved to evade this grapevine immune recognition system.