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Spatial transcriptomes of iron‐deficient and cadmium‐stressed rice
Author(s) -
Ogo Yuko,
Kakei Yusuke,
Itai Reiko Nakanishi,
Kobayashi Takanori,
Nakanishi Hiromi,
Takahashi Hirokazu,
Nakazono Mikio,
Nishizawa Naoko K.
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12577
Subject(s) - transcriptome , biology , vascular bundle , gene expression , gene , homeostasis , microbiology and biotechnology , laser capture microdissection , cadmium , genetics , botany , chemistry , organic chemistry
Summary Although the genes involved in metal homeostasis have been investigated over the past few decades, many genes related to metal homeostasis remain uncharacterized, and a comprehensive analysis of the expression of these genes is required. In the present study, we investigated the spatial gene expression profile of iron (Fe)‐deficient and cadmium (Cd)‐stressed Oryza sativa (rice) using laser microdissection and microarray analysis. Roots of Fe‐deficient and Cd‐stressed rice were separated into the vascular bundle, cortex, and epidermis plus exodermis. In addition, vascular bundles from new and old leaves at the lowest node, which are important for metal distribution, were analyzed separately. The spatial expression patterns were distinct in each tissue type. Fe deficiency and Cd stress also had significant effects on the transcriptomes, although these were less pronounced than the spatial effects. Genes encoding transporters involved in metal homeostasis, proteins associated with heavy metal detoxification, and phytohormone‐related proteins were comprehensively investigated. Additionally, cis motifs involved in the regulation of these diverse expression changes in various tissue types were predicted. The spatial transcriptomes presented here provide novel insight into the molecular mechanisms of metal homeostasis.