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Involvement of the ABCG 37 transporter in secretion of scopoletin and derivatives by Arabidopsis roots in response to iron deficiency
Author(s) -
Fourcroy Pierre,
SisóTerraza Patricia,
Sudre Damien,
Savirón María,
Reyt Guilhem,
Gaymard Frédéric,
Abadía Anunciación,
Abadia Javier,
ÁlvarezFernández Ana,
Briat JeanFrançois
Publication year - 2014
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12471
Subject(s) - arabidopsis , phenylpropanoid , atp binding cassette transporter , secretion , transporter , biochemistry , coumarin , mutant , umbelliferone , chemistry , iron deficiency , arabidopsis thaliana , wild type , biosynthesis , biology , botany , gene , medicine , anemia
Summary Studies of Iron (Fe) uptake mechanisms by plant roots have focussed on Fe( III )‐siderophores or Fe( II ) transport systems. Iron deficency also enhances root secretion of flavins and phenolics. However, the nature of these compounds, their transport outside the roots and their role in Fe nutrition are largely unknown. We used HPLC / ESI ‐ MS ( TOF ) and HPLC / ESI ‐ MS / MS (ion trap) to characterize fluorescent phenolic‐type compounds accumulated in roots or exported to the culture medium of Arabidopsis plants in response to Fe deficiency. Wild‐type and mutant plants altered either in phenylpropanoid biosynthesis or in the ABCG 37 ( PDR 9) ABC transporter were grown under standard or Fe‐deficient nutrition conditions and compared. Fe deficiency upregulates the expression of genes encoding enzymes of the phenylpropanoid pathway and leads to the synthesis and secretion of phenolic compounds belonging to the coumarin family. The ABCG 37 gene is also upregulated in response to Fe deficiency and coumarin export is impaired in pdr9 mutant plants. Therefore it can be concluded that: Fe deficiency induces the secretion of coumarin compounds by Arabidopsis roots; the ABCG 37 ABC transporter is required for this secretion to take place; and these compounds improved plant Fe nutrition.