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Arabidopsis Rop GEF 4 and Rop GEF 10 are important for FERONIA ‐mediated developmental but not environmental regulation of root hair growth
Author(s) -
Huang GuoQiang,
Li En,
Ge FuRong,
Li Sha,
Wang Qun,
Zhang ChunQing,
Zhang Yan
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12432
Subject(s) - root hair , guanine nucleotide exchange factor , arabidopsis , chemistry , microbiology and biotechnology , bimolecular fluorescence complementation , biology , biochemistry , gtpase , mutant , gene
Summary We investigated a genetic pathway in root hair development in Arabidopsis thaliana , involving the receptor‐like kinase FERONIA ( FER ), two guanine nucleotide exchange factors for ROP s (Rop GEF 4 and Rop GEF 10), and the small GTP ase Rho of plants ( ROP s). Loss‐ and gain‐of‐function analyses demonstrated distinct roles of Rop GEF 4 and Rop GEF 10 such that Rop GEF 4 is only important for root hair elongation, while Rop GEF 10 mainly contributes to root hair initiation. Domain dissection by truncation and domain‐swapping experiments indicated that their functional distinctions were mainly contributed by the noncatalytic domains. Using fluorescent ratio imaging, we showed that functional loss of Rop GEF 4 and Rop GEF 10 additively reduced reactive oxygen species ( ROS ) production. Bimolecular fluorescence complementation experiments demonstrated that Rop GEF 4 and Rop GEF 10 had the same interaction specificity as ROP s, suggesting common downstream components. We further showed that the promoting effects of environmental cues such as exogenous auxin and phosphate limitation on root hair development depended on FER . However, although functional loss of Rop GEF 4 and Rop GEF 10 largely abolished FER ‐induced ROS production, it did not compromise the responses to FER ‐mediated environmental cues on root hair development. Our results demonstrated that Rop GEF 4 and Rop GEF 10 are genetic components in FER ‐mediated, developmentally (but not environmentally) regulated, root hair growth.