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The Chinese wild grapevine ( Vitis pseudoreticulata ) E3 ubiquitin ligase Erysiphe necator ‐induced RING finger protein 1 (EIRP1) activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor
Author(s) -
Yu Yihe,
Xu Weirong,
Wang Jie,
Wang Lei,
Yao Wenkong,
Yang Yazhou,
Xu Yan,
Ma Fuli,
Du Yangjian,
Wang Yuejin
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12418
Subject(s) - ubiquitin ligase , biology , ubiquitin , ring finger , powdery mildew , microbiology and biotechnology , pseudomonas syringae , arabidopsis , proteasome , proteolysis , ring finger domain , dna ligase , transcription factor , genetics , gene , botany , biochemistry , zinc finger , mutant , enzyme
Summary Ubiquitin‐mediated regulation responds rapidly to specific stimuli; this rapidity is particularly important for defense responses to pathogen attack. Here, we investigated the role of the E3 ubiquitin ligase Erysiphe necator ‐induced RING finger protein 1 (EIRP1) in the defense response of Chinese wild grapevine Vitis pseudoreticulata . The regulatory function of E3 ubiquitin ligase EIRP1 was investigated using molecular, genetic and biochemical approaches. EIRP1 encodes a C3HC4‐type Really Interesting New Gene (RING) finger protein that harbors E3 ligase activity. This activity requires the conserved RING domain, and VpWRKY11 also interacts with EIRP1 through the RING domain. VpWRKY11 localizes to the nucleus and activates W‐box‐dependent transcription in planta . EIRP1 targeted VpWRKY11 in vivo , resulting in VpWRKY11 degradation. The expression of EIRP1 and VpWRKY11 responds rapidly to powdery mildew in Vitis pseudoreticulata grapevine; also, overexpression of EIRP1 in Arabidopsis confers enhanced resistance to the pathogens Golovinomyces cichoracearum and Pseudomonas syringae pv tomato DC3000. Our data suggest that the EIRP1 E3 ligase positively regulates plant disease resistance by mediating proteolysis of the negative regulator VpWRKY11 via degradation by the 26S proteasome.